Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

نویسندگان

  • F.L.E. Spositto
  • P.A.Z. Campanerut
  • L.D. Ghiraldi
  • C.Q.F. Leite
  • M.H. Hirata
  • R.D.C. Hirata
  • V.L.D. Siqueira
  • R. Fressatti Cardoso
چکیده

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

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عنوان ژورنال:

دوره 45  شماره 

صفحات  -

تاریخ انتشار 2014